A 34-year-old woman from Żejtun was injured late on Monday when cycling at Triq Għar Dalam, near her hometown.
The accident happened at about 7pm when she was involved in a collision with a Toyota Auris car driven by a 23-year-old man from Żejtun.
The woman was assisted by an ambulance crew and later hospitalised in a serious condition.
Police are investigating.